ABS217

Anti-phospho-LAT Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_055202
• UniProt accession no.: O43561
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr200)
• Gene Information: human ... LAT(27040)

Description

General description

LAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.

Specificity

Other homologies: Rat, Mouse, and Bovine (89% sequence homology).

This antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.

Immunogen

Epitope: Cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1

KLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.

Application

Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.

Research Category
Signaling

Research Sub Category
Immunological Signaling

Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.

Quality

Evaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.

Target description

~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.

Linkage

Replaces: 04-467

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable