ABS1680

Anti-Chromatin-Modifying Protein 2a

from rabbit

• Synonym(s): Charged multivesicular body protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_055268
• UniProt accession no.: O43633
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CHMP2A(27243)

Description

General description

Charged multivesicular body protein 2a (UniProt: O43633; also known as Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1) is encoded by the CHMP2A (also known as BC2, CHMP2) gene (Gene ID: 27243) in human. CHMP2a is a core component of the endosomal sorting required for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It localizes to the mid-body of dividing cells. CHMP2a consists of an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. Cells lacking CHMP2A exhibit mitotic defects. They form transient monopolar spindles and fail to segregate chromosomes normally and also fail to complete abscission. Cell death is also reported to be higher in cells depleted of CHMP2A, CHMP4B, and VPS4B.

Specificity

This polyclonal antibody specifically detects human Chromatin-modifying protein 2a.

Immunogen

Epitope: unknown

GST-tagged full-length human recombinant Chromatin-modifying protein 2a.

Application

Anti-Chromatin-Modifying Protein 2a, Cat. No. ABS1680, is a rabbit polyclonal antibody that targets Charged multivesicular body protein 2a and has been tesed for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected Chromatin-Modifying Protein 2a in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94; Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62).

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 2a in 10 µg of HEK293 cell lysate.

Target description

~32 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable