ABN1656
Anti-Stra8 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Stimulated by retinoic acid gene 8 protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
electron microscopy: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... STRA8(346673)
mouse ... Stra8(20899)
General description
Stimulated by retinoic acid gene 8 protein (UniProt P70278) is encoded by the Stra8 gene (Gene ID 20899) in murine species. Stra8 is an early retinoic acid (RA) responsive gene that encodes a cytoplasmic protein expressed exclusively in gonads, where it plays a pivotal role in initiating meiosis of both female and male germ cells. In female mice, Stra8 is expressed in embryonic gonocytes during a restricted time window and is indispensable for their entry into the meiotic S phase. In males, Stra8 expression is first detected in primitive spermatogonia at P5, then becomes prominent in premeiotic (preleptotene) spermatocytes from P10 onwards. Spermatogenesis, the transformation of stem cells into millions of haploid spermatozoa, is a highly regulated process that allows a constant spermatozoa production. Undifferentiated mitotic spermatogonia first develop into committed spermatogonia to produce spermatocytes, which then undergo meiosis to form haploid spermatids. Stra8 is not only required for meiosis, but also promotes spermatogonial differentiation. In the absence of Stra8, spermatogonial differentiation is impaired, giving rise to massive accumulations of undifferentiated and early differentiating spermatogonia (Type A spermatogonia) in aged testes. Stra8-deficient germ cells in postnatal male and fetal female mice arrest just before meiosis, without entering meiotic prophase.
Specificity
This polyclonal antibody detected Stra8 immunoreactivity in testes from wild-type, but not Stra8-knockout mice (Mark, M., et al. (2008). J. Cell Sci. 121(Pt 19):3233-3242).
Immunogen
His-tagged recombinant full-length mouse Stra8.
Application
Anti-Stra8 Antibody, Cat. No. ABN1656, is a highly specific rabbit polyclonal antibody that targets STRA8 and has been tested in Electron Microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Stra8 immunoreactivity in human testis tissue section.
Western Blotting Analysis: 2 µg/mL from a representative lot detected an upregulation of ~35 kDa Stra8 isoform in 10 µg of lysate from retinoid acid-treated P19 murine embryonic carcinoma cells (Courtesy from the laboratory of Dr. Mustapha Oulad-Abdelghani, IGBMC, France).
Electron Microscopy Analysis: A representative lot detected Stra8 immunoreactivity in the cytoplasm of germ cells in direct contact with the basal lamina and displaying features of spermatogonia. No Stra8 immunoreactivity was found in Sertoli cells in mouse testis cryosections (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Immunocytochemistry Analysis: A representative lot detected exogenously expressed murine Stra8 in 2% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized COS-7 transfectants (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Immunofluorescence Analysis: A representative lot detected stage-dependent Stra8 immunoreactivity among A1 A4 differentiated spermatogonia, preleptotene spermatocytes, and in early meiotic spermatocytes by fluorescent immunohistochemistry staining of sections from paraformaldehyde-perfused mouse testis (Mark, M., et al. (2015). Biochim. Biophys. Acta. 1849(2):84-93).
Immunofluorescence Analysis: A representative lot detected Stra8 immunoreactivity in 4% paraformaldehyde-fixed testes sections from wild-type, but not Stra8-knockout mice (Mark, M., et al. (2008). J. Cell Sci. 121(Pt 19):3233-3242).
Immunohistochemistry Analysis: A representative lot detected Stra8 immunoreactivity restricted to cells within the peripheral layer of the tubule of mouse testis cryosections. No Stra8 immunoreactivity was found in Sertoli cells (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Western Blotting Analysis: A representative lot detected an induction of Stra8 expression in multiple phosphorylated forms in P19 murine embryonic carcinoma cells upon stimulation by either all-trans (T-RA) or 9-cis (9C-RA) retinoic acid (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Western Blotting Analysis: 2 µg/mL from a representative lot detected an upregulation of ~35 kDa Stra8 isoform in 10 µg of lysate from retinoid acid-treated P19 murine embryonic carcinoma cells (Courtesy from the laboratory of Dr. Mustapha Oulad-Abdelghani, IGBMC, France).
Electron Microscopy Analysis: A representative lot detected Stra8 immunoreactivity in the cytoplasm of germ cells in direct contact with the basal lamina and displaying features of spermatogonia. No Stra8 immunoreactivity was found in Sertoli cells in mouse testis cryosections (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Immunocytochemistry Analysis: A representative lot detected exogenously expressed murine Stra8 in 2% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized COS-7 transfectants (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Immunofluorescence Analysis: A representative lot detected stage-dependent Stra8 immunoreactivity among A1 A4 differentiated spermatogonia, preleptotene spermatocytes, and in early meiotic spermatocytes by fluorescent immunohistochemistry staining of sections from paraformaldehyde-perfused mouse testis (Mark, M., et al. (2015). Biochim. Biophys. Acta. 1849(2):84-93).
Immunofluorescence Analysis: A representative lot detected Stra8 immunoreactivity in 4% paraformaldehyde-fixed testes sections from wild-type, but not Stra8-knockout mice (Mark, M., et al. (2008). J. Cell Sci. 121(Pt 19):3233-3242).
Immunohistochemistry Analysis: A representative lot detected Stra8 immunoreactivity restricted to cells within the peripheral layer of the tubule of mouse testis cryosections. No Stra8 immunoreactivity was found in Sertoli cells (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Western Blotting Analysis: A representative lot detected an induction of Stra8 expression in multiple phosphorylated forms in P19 murine embryonic carcinoma cells upon stimulation by either all-trans (T-RA) or 9-cis (9C-RA) retinoic acid (Oulad-Abdelghani, M., et al. (1996). J. Cell Biol. 135(2):469-477).
Research Category
Neuroscience
Neuroscience
Quality
Evaluated by Western Blotting in P19 cells.
Western Blotting Analysis: 1 µg/mL of this antibody detected Stra8 expression in 10 µg lysate from all-trans retinoic acid-treated P19 murine embryonic carcinoma cells.
Western Blotting Analysis: 1 µg/mL of this antibody detected Stra8 expression in 10 µg lysate from all-trans retinoic acid-treated P19 murine embryonic carcinoma cells.
Target description
~53/35 kDa observed. 36.91/44.56 kDa (human/mouse; UniProt Q7Z7C7/P70278) calculated. Note: At least five spliced isoforms ranging from 35 to 55 kDa have been identified. Uncharacterized bands may be observed in some lysate(s).
Physical form
Protein A purified.
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Li Li et al.
Cell stem cell, 20(6), 858-873 (2017-05-02)
Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000
Mei Wang et al.
Cell stem cell, 23(4), 599-614 (2018-09-04)
Spermatogenesis generates mature male gametes and is critical for the proper transmission of genetic information between generations. However, the developmental landscapes of human spermatogenesis remain unknown. Here, we performed single-cell RNA sequencing (scRNA-seq) analysis for 2,854 testicular cells from donors
Ke Song et al.
Nature communications, 13(1), 7335-7335 (2022-11-29)
Type 2 diabetes mellitus is one of the most prevalent metabolic diseases presenting with systemic pathologies, including reproductive disorders in male diabetic patients. However, the molecular mechanisms that contributing to spermatogenesis dysfunction in diabetic patients have not yet been fully
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