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662099

Sigma-Aldrich

Anti-Ubiquitin Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

monkey, chicken, guinea pig, frog, yeast, sheep, rabbit, mouse, bovine, canine, E. coli, rat, human, hamster, Drosophila

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UBB(7314)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin.

Immunogen

Bovine
bovine erytherocyte ubiquitin conjugated to KLH

Application

Immunoblotting (1:1000, chemiluminescence)

Immunohistochemistry (1:200)

Immunoprecipitation (1:100)

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following initial thaw, aliquot and freeze (-20°C)

Other Notes

Jungmann, J., et al. 1993. Nature361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem.61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol.4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet.26, 179.
Hershko, A., et al. 1982. J. Biol. Chem.257, 13964.
Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isabelle I Salles et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(21), 12426-12431 (2003-10-02)
In the current study, we show that macrophages adaptively resist anthrax lethal toxin (LT) through a toxin-activated process termed toxin-induced resistance (TIR). TIR was triggered by pretreatment of RAW 264.7 or J774A.1 macrophages with a low dose of LT for
Claudia Castro et al.
The Journal of biological chemistry, 278(7), 4482-4490 (2002-12-13)
Excessive proliferation and migration of vascular smooth muscle cells (SMCs) participate in atherosclerotic plaque growth. In this study, we investigated whether SMCs from vessels with different atherogenicity exhibit distinct growth and migratory potential and investigated the underlying mechanisms. In fat-fed
Roi Cal et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(2), 369-377 (2012-12-18)
Low density lipoprotein retention and aggregation in the arterial intima are key processes in atherogenesis. Aggregated LDL (agLDL) is taken up through low-density lipoprotein receptor-related protein 1 (LRP1) by human vascular smooth muscle cells (VSMC). AgLDL increases LRP1 expression, at
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Syed Feroj Ahmed et al.
Science advances, 6(34) (2020-09-17)
Cross-talk between ubiquitination and ADP-ribosylation regulates spatiotemporal recruitment of key players in many signaling pathways. The DELTEX family ubiquitin ligases (DTX1 to DTX4 and DTX3L) are characterized by a RING domain followed by a C-terminal domain (DTC) of hitherto unknown
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