539660
PKCε, Human, Recombinant, S. frugiperda
Synonym(s):
Protein Kinase Cε
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About This Item
Assay
≥85% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥800 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full-length, recombinant, human, PKCε expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCε and its regulation. PKCε is a Ca2+-independent and phospholipid-dependent enzyme.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.4.
Physical form
In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.
Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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