1.50479
SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns
L × I.D. 150 mm x 0.3 mm capillay Column
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41115709
Recommended Products
product name
SeQuant® ZIC-HILIC™ (3.5µm,200Å) 150 x 0.3 mm, suitable for HPLC
material
stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
400 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)
column L × I.D.
15 cm × 0.3 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
Related Categories
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Application
- Click postsynthesis of microporous organic network@silica composites: This study presents the creation of novel composites for mixed-mode chromatography, showcasing an innovative approach to chemical separation technologies, which includes hydrophilic interaction liquid chromatography (HILIC) (Sun HF et al., 2023).
- Evaluation of Hold-Up Volume Determination Methods: This research evaluates different methods and markers in HILIC, critical for refining analytical methods in complex sample matrices such as pharmaceuticals, enhancing accuracy and reproducibility in chemical analysis (Redon L et al., 2023).
- Determination of polyphenolic content in berry juices: Utilizing focusing-modulated comprehensive two-dimensional liquid chromatography, this study highlights the application of HILIC in food analysis, demonstrating its effectiveness in extracting detailed nutritional information (Arena K et al., 2023).
- Simple and sensitive determination of sulfites in Chinese herbal teas: This paper employs ultra-high-performance liquid chromatography coupled with mass spectrometry, showing HILIC′s role in ensuring consumer safety by detecting potentially harmful additives in herbal products (Yang L et al., 2022).
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service