1.19612

Superspher^® 60 RP-8 (4 µm) HPLC Columns

HPLC sorbent

• Synonym(s): TM=["Superspher"] C8 sorbent for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Superspher^® 60 RP-8,
• material: stainless steel column
• Agency: suitable for USP L7
• product line: Superspher^®
• feature: endcapped: no
• parameter: 60 °C max. temp.
• technique(s): HPLC: suitable; mass spectrometry (MS): suitable (Low - Medium)
• matrix: fully porous particle
• matrix active group: C8 bonding phase
• particle size: 4 μm
• pore size: 60 Å pore size
• operating pH range: 2-7.5
• bulk density: 200‑400 kg/m³
• separation technique: reversed phase
• storage temp.: no temp limit

Description

Application

• An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC: This study demonstrates the use of Superspher 60 RP-8 for the high-resolution separation of amino acid derivatives, crucial in biochemical analysis of protein structures. Optimized HPLC protocols improve analytical accuracy and efficiency, beneficial for fields such as analytical chemistry and pharmaceutical analysis (Persson B, Eaker D, 1990).

Analysis Note

Application test: passes test
Theoretical Plates (N/m) (Anthracene): ≥ 105000
stability testing: passes test
selectivity test: passes test
separation factor (Lysine/Digitoxigenine): 1.6 - 2.1
separation factor (Lysine/PHB-propylester): 1.1 - 1.4
separation factor (Naphthalene/Digitoxigenin, selectivity test): 4.5 - 6.5
capacity factor (naphthalene): 17.0 - 22.0
Particle Size (d10): 3.4 - 4.0 µm
Particle Size (d50): 4.6 - 5.3 µm
Particle Size (d90): 6.4 - 7.2 µm

Legal Information

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable