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05-944

Sigma-Aldrich

Anti-Ubiquitin Antibody, clone P4D1-A11

clone P4D1-A11, Upstate®, from mouse

Synonym(s):

Anti-Ubiquitin, Clone P4D1-A11 Anti-Ubiquitin, Ubiquitin Detection Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P4D1-A11, monoclonal

species reactivity

Saccharomyces cerevisiae, human, vertebrates

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_018955

UniProt accession no.

P62988

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

Specificity

Ubiquitin, Mr = 8 kDa, and several ubiquitinated proteins

Immunogen

total bovine ubiquitin

Application

Anti-Ubiquitin Antibody, clone P4D1-A11 is an antibody against Ubiquitin for use in WB.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Quality

Routinely evaluated by immunoblot of human recombinant ubiquitin (Catalog # 12-558), and ubiquitinated proteins in acid extracts from HeLa cells

Target description

8 kDa and higher

Linkage

Replaces: 04-454

Physical form

100 μg of protein G purified mouse IgG in 100 μL of 70% storage buffer (0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide) and 30% glycerol.
Format: Purified
Protein G Purified

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Osmotic stress changes the expression and subcellular localization of the Batten disease protein CLN3.
Getty, A; Kovacs, AD; Lengyel-Nelson, T; Cardillo, A; Hof, C; Chan, CH; Pearce, DA
Testing null
Regulatory monoubiquitination of phosphoenolpyruvate carboxylase in germinating castor oil seeds.
Uhrig, RG; She, YM; Leach, CA; Plaxton, WC
The Journal of Biological Chemistry null
Regulating the regulators: control of protein ubiquitination and ubiquitin-like modifications by extracellular stimuli.
Gao, Min and Karin, Michael
Molecular Cell, 19, 581-593 (2005)
The ubiquitin-proteasome pathway and its role in cancer.
Mani, Aparna and Gelmann, Edward P
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 23, 4776-4789 (2005)
Assaying protein ubiquitination in Saccharomyces cerevisiae.
Laney, Jeffrey D and Hochstrasser, Mark
Test, 351, 248-257 (2002)
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