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05-809

Sigma-Aldrich

Anti-trimethyl (Lys9)-phospho (Ser10)-Histone H3 Antibody, rabbit monoclonal

Upstate®, from rabbit

Synonym(s):

H3K9me3S10P, Histone H3 (tri methyl K9, phospho S10)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10), trimethylation (Lys9)

Gene Information

human ... H3C1(8350)

Specificity

Broad species cross-reactivity is expected
trimethyl (Lys9)-phospho (Ser10)-histone H3

Immunogen

KLH-conjugated, synthetic peptide containing the sequenc...ARme3KpS... in which me3K corresponds to trimethyl-lysine 9 and pS to phosphoserine 10 of human histone H3

Application

Anti-trimethyl (Lys9)-phospho (Ser10)-Histone H3 Antibody is a rabbit monoclonal antibody for detection of trimethyl (Lys9)-phospho (Ser10)-Histone H3 also known as H3K9me3S10P, has been validated in WB, PIA, Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Target description

Mr 17kDa

Physical form

0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%
Format: Purified
Protein A purified

Storage and Stability

Stable for 2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Scott B Rothbart et al.
Molecular cell, 59(3), 502-511 (2015-07-28)
Access to high-quality antibodies is a necessity for the study of histones and their posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone
Meng Wang et al.
Cancer research, 74(10), 2825-2834 (2014-03-22)
Therapeutics that target the epidermal growth factor receptor (EGFR) can enhance the cytotoxic effects of ionizing radiation (IR). However, predictive genomic biomarkers of this radiosensitization have remained elusive. By screening 40 non-small cell lung cancer cell (NSCLC) lines, we established

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