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Sigma-Aldrich

Anti-IQGAP1 Antibody, clone AF4

clone AF4, Upstate®, from mouse

Synonym(s):

Anti-HUMORFA01, Anti-SAR1, Anti-p195

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

AF4, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003870

UniProt accession no.

P46940

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IQGAP1(8826)

Specificity

IQGAP1, not IQGAP2

Immunogen

A Glu-Glu tagged C-terminal fragment corresponding to amino acids 863-1657 of human IQGAP1, purified from baculovirus infected insect cells

Application

Detect IQGAP1 using this Anti-IQGAP1 Antibody, clone AF4 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

routinely evaluated by immunoblot on HepG2 RIPA cell lysate

Target description

185kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein G Chromatography

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mireille Lambert et al.
The Journal of cell biology, 157(3), 469-479 (2002-04-24)
Cadherin receptors are key morphoregulatory molecules during development. To dissect their mode of action, we developed an approach based on the use of myogenic C2 cells and beads coated with an Ncad-Fc ligand, allowing us to mimic cadherin-mediated adhesion. We
Yuehong Cui et al.
OncoTargets and therapy, 10, 627-643 (2017-02-23)
In recent years, understanding of the role of asparaginyl endopeptidase (AEP) in tumorigenesis has steadily increased. In this study, we investigated whether AEP expression correlates with sensitivity to chemotherapeutic drugs in gastric cancer and explored the mechanism. AEP expression in
Kai-Min Lin et al.
Journal of virology, 97(8), e0054023-e0054023 (2023-07-28)
Epstein-Barr virus (EBV) is a human oncogenic γ-herpesvirus that establishes persistent infection in more than 90% of the world's population. EBV has two life cycles, latency and lytic replication. Reactivation of EBV from latency to the lytic cycle is initiated
Binding of myosin essential light chain to the cytoskeleton-associated protein IQGAP1.
Weissbach, L, et al.
Biochemical and biophysical research communications, 251, 269-276 (1998)
IQGAP1 involvement in MTOC and granule polarization in NK-cell cytotoxicity.
Namita Kanwar,John A Wilkins
European Journal of Immunology null
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