The usage instructionsis as follows:
1. Calculate the desired volume of hydrogel (ECM + cells).
2. Multiply the desired volume by 0.02. This will be the amount of mL of Ruthenium and Sodium Persulfate (each) to be added to the pre-hydrogel solution.
3. Pre-solubilize the required Ruthenium in water or 1X PBS at a concentration of 37.4 mg/mL.
4. Pre-solubilize the Sodium Persulfate in water or 1X PBS at a concentration of 119 mg/mL.
5. Add the calculated volume (from step 2) of Ruthenium to the pre-hydrogel solution and mix thoroughly.
6. Add the calculated volume (from step 2) of Sodium Persulfate to the pre-hydrogel solution and mix thoroughly.
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About This Item
UNSPSC Code:
12162002
NACRES:
NA.23
Recommended Products
composition
Ruthenium (200 mg)
Sodium persulfate photoinitiator (1 g)
Quality Level
Related Categories
Application
Ruthenium is a photoinitiator that utilizes visible light photocrosslinking (400-450nm) to covalently crosslink free tyrosine and acryl groups. Ruthenium photoinitiator has been tested on collagen type I, gelatin, silk fibroin, methacrylated hyaluronic acid, methacrylated gelatin, methacrylated collagen type I and PEGDA. Ruthenium is water soluble and yields better cytocompatibility, and crosslinking efficiency. Ruthenium is red/yellow/orange in color and will change the color of your solutions, hydrogels, or printed constructs. The Ruthenium photoinitiator kit is non-sterile. Adding antibiotics to your cell culture system, or sterile filtering is recommended. To sterile filter, resuspend the entire volume of ruthenium and Sodium persulfate (separately) and filter through small 0.2 micron button filters (separately). Use the sterile photoinitiator within 2 weeks. Ruthenium photoinitiator kit is ideal for tissue engineering, cell culture, and bioprinting, where tuning the mechanical properties of the substrate is required. The kit provides enough photoinitiator for >200 mL of bioinks/hydrogels.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
5.1B - Oxidizing hazardous materials
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What are the steps to mix together and utilize product no. 916811-1EA, the Ruthenium photoinitiator kit?
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