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760765

Sigma-Aldrich

Azide-fluor 488

≥90% (HPLC)

Synonym(s):

Rhodamine 110-azide

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1 MG
€88.50
5 MG
€332.00

€88.50


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1 MG
€88.50
5 MG
€332.00

About This Item

Empirical Formula (Hill Notation):
C29H30N6O7
Molecular Weight:
574.58
UNSPSC Code:
12352125
NACRES:
NA.22

€88.50


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

Assay

≥90% (HPLC)

form

solid

reaction suitability

reaction type: click chemistry

storage temp.

−20°C

Application

Rhodamine 110 conjugated PEG azide. Can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.

Spectral Properties: Abs/Em = 501/525 nm

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Chemical tools for studying the biological function of glycolipids.
Stocker BL & Timmer MS
Chembiochem, 14(10), 1164-1184 (2013)
Two-step local functionalization of fluoropolymer Dyneon THV microfluidic materials by scanning electrochemical microscopy combined to click reaction.
Slim C, et al.
Electrochemical Communications, 60, 5-8 (2015)
Synthesis and Conjugation of Alkyne-Functional Hyperbranched Polyglycerols.
Moore E, et al.
Macromolecular Chemistry and Physics, 217(20), 2252-2261 (2016)
Quantification and visualization of glutathione S-transferase omega 1 in cells using inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence microscopy
Liang Y, et al.
Analytical and Bioanalytical Chemistry, 407(9), 2373-2381 (2015)
Drawing in poly (e-caprolactone) fibers: tuning mechanics, fiber dimensions and surface-modification density
Kim SE, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine (2017)

Articles

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

Questions

1–3 of 3 Questions  
  1. After resuspending the Rho-azide (#760765) in DMF, I observed a color change to fluorescent yellow, whereas reconstitution in PBS buffer retains its pink color. Could this color change potentially affect the results of the click-chemistry?

    1 answer
    1. This product is not tested for solubility. Thus, the color change of the solution from solvent to solvent has not been investigated. However, the polarity of a given solvent may impact the color of the resulting solution. The color change is very unlikely to be related to click chemistry as DMF lacks an appropriate function group.

      Helpful?

  2. After reconstituting product 760765, should it still be stored at -20 degrees Celsius?

    1 answer
    1. The solution stability of this product has not been determined. However, various sources suggest that this material may be stored for up to one month at -20°C or six months at -80°C in organic solvents such as DMSO or DMF. Solutions prepared in water are less stable and should be used within several hours. Note that this information has not been validated.

      Helpful?

  3. Hello, why is this labeled as fluor 488 when it is a Rhodamine 110 and not an Alexa Fluor 488 fluorophore?

    1 answer
    1. As listed on the product detail page, Rhodamine 110-azide is a synonym of Azide-fluor 488.

      Helpful?

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