Phosphorylated peptides are attractive targets in the study of the phosphoproteome. Here, we introduce a simple and convenient micropipette-tip method for the separation of phosphorylated and nonphosphorylated peptides by using a phosphate-binding zinc(II) complex of 1,3-bis(pyridin-2-ylmethylamino)propan-2-olate (Phos-tag). A 200-μL micropipette
Okadaic acid (OKA), a marine toxin produced by dinoflagellates, is responsible for most human diarrhetic shellfish poisoning-associated health disorders. A competitive displacement assay for OKA is described here. An OKA-binding aptamer was truncated with two sequences, one labeled with 6-carboxyfluorescein
The development of ruthenium-based complexes for cancer treatment requires a variety of pharmacological studies, one of them being a drug's binding kinetics to serum proteins. In this work, speciation analysis was used to study kinetics of ruthenium-based drug candidates with
Physical chemistry chemical physics : PCCP, 21(18), 9564-9573 (2019-04-26)
An interconnected graphene network (IGN) structure with excellent photoluminescence (PL) properties was synthesized using a one-pot microwave-assisted hydrothermal carbonization route. The material exhibited intense and excitation-wavelength dependent PL emission located mainly in the UV-blue light range (300-450 nm). The result
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