QR0100
SYBR® Green Quantitative RT-qPCR Kit
One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase
Synonym(s):
one step rt qPCR, sybr green qPCR
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€902.00
Availability
Select a Size
About This Item
€902.00
Availability
Recommended Products
Quality Level
usage
sufficient for 250 reactions
feature
dNTPs included
hotstart
technique(s)
RT-qPCR: suitable
color
colorless
input
purified RNA
compatibility
ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | S9194 | KCQS00 | KCQS01 |
---|---|---|---|
sufficient for 250 reactions | sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions | sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions |
dNTPs included | dNTPs included, hotstart | dNTPs included, hotstart | dNTPs included, hotstart |
colorless | colorless | colorless | colorless |
purified RNA | purified DNA | purified DNA | purified DNA |
ABI 5700 | for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus | for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480 | for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000 |
General description
Application
Features and Benefits
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced preparation time and the risk of contamination from multiple pipetting steps
- Reduced set-up time as compared to manual or wax Hot Start methods
- JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Broad instrument compatibility
- Includes a separate ROX reference dye vial for reaction normalization
Packaging
Legal Information
Kit Components Only
- SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Protocols
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
Related Content
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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