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Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR

Synonym(s):

BHQ, BHQ Probes, Black Hole Quencher, Dual-Quenched Probes,, Internal-Quenched Probes, Locked Nucleic Acid probes, MGB:EDQ Probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs

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About This Item

UNSPSC Code:
41105904




 

Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid and MGB:EDQ available
  • Dyes: Comprehensive offering of reporters and 3' / internal / dual quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

 


Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Locked Nucleic Acid & MGB:EDQ

  • Available with Dual-Labeled Probes and other oligos
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more

Black Hole Quencher™ (BHQ™)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more
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Articles

Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments.

If you commercialize Molecular Diagnostics (MDx), develop Laboratory Developed Tests (LDTs), or conduct preclinical research on therapeutics, then your final kit, assay, or modality must have an extraordinary level of quality to comply with strict regulatory or study standards.

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

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Global Trade Item Number

SKUGTIN
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