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G005330

HPLC Analysis of Alanine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Modifier)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 205 nm
injection: 10 μL
sample: 300 μg /mL in 30:70 water:methanol

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    L-Alanine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
    D-Alanine, ≥98% (HPLC)

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