56400AST

apHera^™ NH₂ (5 μm) HPLC Columns

L × I.D. 15 cm × 2 mm, HPLC Column

• Synonym(s): Amino Functionalized HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: apHera^™ NH₂ HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2 mm
• material: stainless steel column
• Agency: suitable for USP L82
• product line: apHera^™
• packaging: pkg of 1 ea
• manufacturer/tradename: apHera^™
• parameter: 147 bar pressure (2133 psi); 4-50 °C temperature
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 2 mm
• matrix: polymeric
• matrix active group: amino phase
• particle size: 5 μm
• pore size: 300 Å
• operating pH: 1-14
• application(s): food and beverages
• separation technique: hydrophilic interaction (HILIC); normal phase

Description

General description

apHera^™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera^™ Columns

• High efficiency for carbohydrate analysis
• Ideal for basic conditions/amphoteric detection
• High selectivity mono- to oligosaccharides
• pH range 2-13
• 300 angstrom pore size
• Stable, predictable retention
• Long column life

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC