ZRB1478

Anti-RBM39 Antibody, clone 1H21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): RNA-binding protein 39;CAPER alpha;CAPERalpha;Hepatocellular carcinoma protein 1;RNA-binding motif protein 39;RNA-binding region-containing protein 2;Splicing factor HCC1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1H21, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 59.38 kDa; observed mol wt ~65 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): porcine, monkey, rat, bovine, feline, canine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_909122
• UniProt accession no.: Q14498
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RBM39(9584)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects RNA-binding protein 39 (RBM39). It targets an epitope within 22 amino acids from the C-terminal half

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human RNA-binding protein 39 (RBM39).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RBM39 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RBM39 in Neuro 2A cell lysate.

Western Blotting Analysis(WB): 1:1000 dilution of this antibody detected RBM29 in wild type HCT116 cells, but not in lysates from cells treated with Indisulam.

Western blotting Analysis: 1:1000 dilution of this antibody detected RBM39 in lysate from thymus tissue of heterozygous knockout mice, but not from lysate from homozygous knockout mice.

Affinity Binding Assay: A representative lot of this antibody bound RBM39 peptide with a KD of 3.9 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RBM39 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RBM39 in HeLa cell lysate.

Target description

RNA-binding protein 39 (UniProt: Q14498; also known as CAPER alpha, CAPERalpha, Hepatocellular carcinoma protein 1, RNA-binding motif protein 39, RNA-binding region-containing protein 2, Splicing factor HCC1) is encoded by the RBM39 (also known as HCC1, RNPC2) gene (Gene ID: 9584) in human. RBM39 is an RNA-binding protein that acts as a pre-mRNA splicing factor. It is mainly involved in transcriptional regulation, alternative splicing, and protein translation. It is normally concentrated in nuclear speckles and has three RNA recognition motifs (RRM)(aa 153-230; 250-328; and 445-508). RBM39 is considered to be a key factor in tumor-targeted mRNA and protein expression and plays a vital role in tumorigenesis. It is expressed at high levels in human breast cancer tissues, but it was almost undetectable in normal breast tissues. Its levels are reported to be upregulated in most cancers and its inhibition is lethal to cancer cells. By regulating transcription of many tumor-related genes, protein translation, and alternative splicing it is indirectly involved in tumor growth and progression. It displays high selectivity for c-Jun, ER /β, and progestin receptor (PR), but does not affect other transcription factors, such as p53, c-fos, NF- B component p50 etc. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Xu, C., et al. (2021). Cell Death Dis. 7; Article 214; Chai, Y., et al. (2014). Tumor Biol. 35(7); 6311-6317).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable