SAB4200497

Anti-THY1 antibody, Mouse monoclonal

clone TH350, purified from hybridoma cell culture

• Synonym(s): Anti-CD90, Anti-CDw90, Anti-FLJ33325, Anti-Thy-1 T-cell antigen, Anti-Thy-1 antigen, Anti-Thy-1 cell surface antigen, Anti-thy-1 membrane glycoprotein, Monoclonal Anti-THY1 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: TH350, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~27 kDa
• species reactivity: mouse, human, rat
• concentration: ~1.0 mg/mL
• technique(s): ELISA: suitable; flow cytometry: 20-40 μg/test using A549 cells.; immunohistochemistry: suitable; western blot: 2.0-4.0 μg/mL using NT2 total cell extracts.
• UniProt accession no.: P04216
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... THY1(7070)

Description

General description

Monoclonal Anti-THY1 (mouse IgG1 isotype) is derived from the hybridoma TH350 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to keyhole limpet hemocyanin (KLH). Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.

THY-1 is expressed in various cell types, including human fibroblasts, neurons, blood stem cells and endothelial cells as well as murine T cells.

Specificity

Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to KLH.

Application

Monoclonal Anti-THY1 antibody produced in mouse may be used in:

• immunoblotting
• flow cytometry
• immunofluorescence

Biochem/physiol Actions

THY-1 is also involved in the inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration and fibroblast proliferation and migration. This protein plays a role as a mediator of cell-cell and cell-matrix interactions. Thy-1 is a cell surface marker in identifying local and circulating liver cancer stem cells. This protein′s expression can be regulated by the epigenetic mechanism of promoter region methylation, in which down-regulation of Thy-1 is associated with a more invasive/metastatic clinical phenotype.

Thy-1 cell surface antigen (THY1) has a role in the activation of T-cells, release of neurotransmitters at synapses, enhancement of adhesion of astrocytes, wound healing, fibrogenesis and tumor suppression. It also plays an important role in cell migration and cytoskeletal reorganization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable