immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon. indirect immunofluorescence: 1-2 μg/mL using MDCK cells western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells
Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).
Immunogen
a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.
Application
Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.
Biochem/physiol Actions
Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin
The Journal of Cell Biology, 141(7) (1998)
Recessive Mutations in the Gene Encoding the Tight Junction Protein Occludin Cause Band-like Calcification with Simplified Gyration and Polymicrogyria
Mary C, et al.
American Journal of Human Genetics (2010)
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Mitinouri Saitou, et al
Molecular Biology of the Cell (2017)
Splicing Diversity of the Human OCLN Gene and Its Biological Significance for Hepatitis C Virus Entry? ?
Indu Kohaar, et al.
Molecular and Cellular Biology (2010)
Analyses of Gingival Adhesion Molecules in Periodontitis: Theoretical In Silico, Comparative In Vivo, and Explanatory In Vitro Models
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