S7901

Stem Cell Factor human

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): SCF, c-Kit ligand
• MDL number: MFCD00165757
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: powder
• potency: 1-5 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: protein 18.5 kDa
• packaging: pkg of 10 μg; pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μgtested (LAL test)
• UniProt accession no.: P21583
• storage temp.: −20°C
• Gene Information: human ... KITLG(4254)

Description

Application

Stem Cell Factor human has been used in

• In vitro differentiation of equine embryonic stem cells
• Chemotaxis detection system
• Ex vivo expansion of umbilical cord blood CD34⁺ cells
• Cytokine therapy

Biochem/physiol Actions

Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves