S5439

Src human

≥80% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution, histidine-tagged

• MDL number: MFCD03458574
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: 60 kDa
• concentration: ~0.58 mg/mL protein (Bradford assay)
• UniProt accession no.: P12931
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SRC(6714)

Description

General description

SRC proto-oncogene, non-receptor tyrosine kinase (SRC) belongs to the family of non-receptor tyrosine kinases. It contains an amino-terminal domain, conserved Src-homology domains (SH2 and SH3), a tyrosine kinase domain and the carboxyl-terminus. The gene encoding this protein is localized on human chromosome 20q11.23.

Application

Human Src has been used to study the kinetics of Src entrapped in a series of different sol-gel derived materials.

Biochem/physiol Actions

SRC proto-oncogene, non-receptor tyrosine kinase (SRC) phosphorylates membrane proteins and nucleoproteins and activates various intracellular signaling cascades. It has been associated with a number of human malignancies and cancers.

Unit Definition

One unit will phosphorylate one nmole of poly(Gly:Tyr) 4:1 per minute at pH 7.5 at 30°C.

Physical form

Solution in 50 mM Tris pH 7.5, 0.05 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.05% NP-40, and 50% glycerol.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable