S3887
Streptavidin–β-Galactosidase conjugate
streptavidin 1-2 units/mg protein, lyophilized powder
Synonym(s):
Streptavidin–beta-Galactosidase conjugate
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tag
beta-galactosidase tagged
Quality Level
form
lyophilized powder
streptavidin activity
1-2 units/mg protein
composition
Protein, ≥80% biuret
storage temp.
−20°C
General description
Streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii, is a very high affinity and specificity non-covalent biotin binding protein that is used in a wide range of biochemical/immunological assays such as western blot and ELISAs. It is a component of the streptavidin-biotin system; wherein biotin is conjugated to a target molecule that is later detected by a signal tagged streptavidin. Streptavidin may be covalently tagged with a range of enzymes that develop chromogenic sustrates or fluorescent molecules to support detection systems.
β-galactosidase may be used in genetic, molecular and immunologic detection systems. Active β-galatosidase cleaves the detection molecule X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) to form an intense blue product that may be detected by chromogenic techniques.
β-galactosidase may be used in genetic, molecular and immunologic detection systems. Active β-galatosidase cleaves the detection molecule X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) to form an intense blue product that may be detected by chromogenic techniques.
Specificity
Streptavidin–β-Galactosidase conjugate is reactive with biotin labeled molecules.
Application
Streptavidin–β-Galactosidase conjugate may be used in conjunction with biotin labeled molecules in techniques such as ELISA, immunohistochemistry, protein blotting or with biotinylated DNA probes. It provides a convenient yet highly sensitive and specific detection system.
Streptavidin–ß-Galactosidase conjugate has been used in the fusion assay in chinese hamster ovary cells.[1]
Streptavidin–ß-Galactosidase conjugate has been used in the fusion assay in chinese hamster ovary cells.[1]
Physical form
Contains Tris buffer salts.
Preparation Note
Streptavidin and β-galactosidase grade VIII are conjugated by a modification of the method of O′Sullivan and purified by gel filtration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan
Febs Letters, 95 (1978)
Human herpesvirus 8 glycoprotein B (gB), gH, and gL can mediate cell fusion
Pertel PE
Journal of Virology, 76, 4390-4400 (2002)
R S Niedbala et al.
Journal of biochemical and biophysical methods, 13(4-5), 205-210 (1986-11-01)
Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)
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