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R9379

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~70,000

Synonym(s):

RITC-Dextran

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About This Item

MDL number:
UNSPSC Code:
12171501
NACRES:
NA.56
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mol wt

average mol wt ~70,000

extent of labeling

0.002-0.015 mol RITC per mol glucose

storage temp.

2-8°C

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General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye.[1]

Application

Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.
Rhodamine B isothiocyanate-Dextran has been used:
  • to label A, CI-M6PR-GFP, Lamp1-CFP-expressing wild type mouse embryonic fibroblast (MEF) cells[2]
  • in endosome/phagosome fusion assay to determine the rate of fusion of endosomes[3]
  • as a component in poration medium to monitor Hela cell uptake[4]

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.[1]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions  
  1. What is the solubility of product R9379, Rhodamine B isothiocyanate–Dextran?

    1 answer
    1. The product is soluble in water at 10mg/mL.

      Helpful?

  2. detection wavelength 알려주세요. 이 제품 production information sheet가 없네요.

    1 answer
    1. For R9379, Rhodamine B isothiocyanate-Dextran, the RITC has excitation and emission wavelengths reported at λex 553 nm; λem 627 nm in methanol.

      Helpful?

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