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R8654

Sigma-Aldrich

Anti-RAD17 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-S.cerevisiae homolog of RAD24, Anti-hRad17

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About This Item

MDL number:
MFCD08705511
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 75 kDa

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000 using HeLa cell lysates

UniProt accession no.

O75943

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAD17(5884)
mouse ... Rad17(19356)
rat ... Rad17(310034)

General description

Cell cycle checkpoint protein RAD17 is mapped to human chromosome 5q13.2 . It is also called s a replication factor C (RFC)-like DNA damage sensor protein. Eight alternative spliced transcript variants of the gene, which encode four distinct proteins, have been reported.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human RAD17 (isoform 1), conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide differs from that of isoform 2 by two amino acids.

Application

Anti-RAD17 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

RAD17 has been reported in breast, non-small cell lung carcinoma and breast cancer. RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. Treatment of human cells with genotoxic agents induced ATM/ATR dependent phosphorylation of RAD17 at Ser635 and Ser645 , suggesting that phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells.

Physical form

Supplied as a solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues
Wang X, et al.
Cancer research, 61(20), 7417-7421 (2001)
Basal-like Breast cancer DNA copy number losses identify genes involved in genomic instability, response to therapy, and patient survival
Weigman VJ, et al.
Breast Cancer Research and Treatment, 133(3), 865-880 (2012)
Rad17 recruits the MRE11-RAD50-NBS1 complex to regulate the cellular response to DNA double-strand breaks
Wang Q, et al.
The Embo Journal, 33(8), 862-877 (2014)
Yasunori Fukumoto et al.
The Journal of biological chemistry, 297(2), 100831-100831 (2021-06-27)
The ATR pathway is one of the major DNA damage checkpoints, and Rad17 is a DNA-binding protein that is phosphorylated upon DNA damage by ATR kinase. Rad17 recruits the 9-1-1 complex that mediates the checkpoint activation, and proteasomal degradation of
Eun Kyeong Oh et al.
International journal of oncology, 41(6), 2038-2046 (2012-10-02)
Host genomic alterations in addition to human papillomavirus (HPV) are needed for cervical precursor lesions to progress to invasive cancer because only a small

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