R2779

Renin human

BioUltra, recombinant, expressed in mammalian cells, ≥90 unit/μg protein, ≥98% (SDS-PAGE)

• Enzyme Commission number: 3.4.23.15 (BRENDA, IUBMB)
• MDL number: MFCD00132171
• UNSPSC Code: 12352204

Properties

• recombinant: expressed in mammalian cells
• product line: BioUltra
• Assay: ≥98% (SDS-PAGE)
• specific activity: ≥90 unit/μg protein
• UniProt accession no.: P00797
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... REN(5972)

Description

Application

Human renin is a member of the Peptidase A1 family. It catalyzes the hydrolysis of angiotensinogen to produce angiotensin I. It is used to study human renin-dependent hypertension as well as renin induced obesity .

Biochem/physiol Actions

Renin is a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. Renin, an aspartyl protease, cleaves angiotensinogen which results in Ang I. Ang I is converted to Ang II by the Ang-converting enzyme (ACE) . Prorenin is hydrolyzed by plasma kallekrein to produce active renin. The human renin transgene is thought to be responsible for increased appetite, obesity, and metabolic changes which are not related to angiotensin II .

Physical properties

Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds.

Supplied as a solution in 50 mM Tris HCl, pH 8

Unit Definition

One unit will produce one fluorescent unit from the hydrolysis of Renin Substrate I (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) at 37 °C at pH 8.0

Preparation Note

Expressed in mammalian cells as prorenin. The prorenin is activated to renin using trypsin and re-purified.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves