R106-05N

Rat Dermal Fibroblasts: RDF, neonatal

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: rat dermis
• Quality Level: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• morphology: Fibroblast
• technique(s): cell culture | mammalian: suitable
• storage temp.: −196°C

Description

General description

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RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).

Cell Line Origin

Skin

Application

Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.

Components

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

• 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RDF Culture Protocol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable