P4406

Papain Agarose from papaya latex

lyophilized powder, 90-150 units/mL packed gel

• MDL number: MFCD00131791
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: papaya (latex)
• form: lyophilized powder
• specific activity: 90-150 units/mL packed gel
• mol wt: 21 kDa
• extent of labeling: 3,000-5,000 units per g agarose (at pH7.0 at 30 °C.)
• matrix: beaded agarose
• storage temp.: −20°C

Description

Application

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below.

Physical form

Lyophilized powder, stabilized with lactose

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves