O7764

Anti-β-O-Linked N-Acetylglucosamine antibody, Mouse monoclonal

clone CTD110.6, purified from hybridoma cell culture

• Synonym(s): Anti-O-GLcNAc, Anti-OGT
• MDL number: MFCD07370422
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CTD110.6, monoclonal
• form: buffered aqueous solution
• species reactivity: wide range
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.2-0.4 μg/mL using total cell extract of HeLa cells
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... OGT(8473)

Description

General description

Monoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH. This antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross react with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G. O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has 13.5 tetratricopeptide-repeats (TPR) and a globular catalytic domain. The gene encoding this protein is localized on human chromosome Xq13.1.

Specificity

The antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross reacts with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.

Immunogen

synthetic peptide YSPTS(O-GlcNAc)PSK.

Application

Monoclonal Anti-β-O-Linked N-Acetylglucosamine antibody produced in mouse has been used in Western Blotting.

Biochem/physiol Actions

O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has a role in the addition of O-linked N-acetylglucosamine (O-GlcNAc) to serine/threonine residues in the protein of interest. It is crucial for O-GlcNAcylation of RNA polymerase II. Absence of OGT has been shown to reduce transcription and pol II promoter occupancy. The gene is upregulated in breast cancer tissues, colon cancer, lung cancer and laryngeal cancer. This protein might be associated with tumorigenesis and metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable