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MAK086

Sigma-Aldrich

Acetate Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle.[1] Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins.[2][2] Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

Application

Acetate colorimetric assay kit has been used to determine acetate concentrations calorimetrically.[3][4]

Suitability

Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food.

Principle

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

replaced by

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
8L11K06580
8L08K06580
8L11K06580
8B14K06580
8D15K06580

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Sheng Li et al.
Bioresource technology, 239, 285-293 (2017-05-23)
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO
A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.
Imam S, et al.
The Plant Journal, 84(6), 1239-1256 (2015)
Acetyl Coenzyme A: A Central Metabolite and Second Messenger
Federico P, et al.
Cell Metabolism, 21(6), 805-821 (2015)
Jeremy D Osko et al.
Biochemistry, 58(36), 3755-3766 (2019-08-23)
Polyamines are small organic cations that are essential for cellular function in all kingdoms of life. Polyamine metabolism is regulated by enzyme-catalyzed acetylation-deacetylation cycles in a fashion similar to the epigenetic regulation of histone function in eukaryotes. Bacterial polyamine deacetylases
A Pichia anomala Strain (P. anomala M1) Isolated from Traditional Greek Sausage is an Effective Producer of Extracellular Lipolytic Enzyme in Submerged Fermentation.
Papagianni M and Emmanuel M P
Ferroelectr., Lett. Sect., 3(3), 43-43 (2017)
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Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the minimum detection limit of this assay? What is the quantification range? 

    1 answer

    1. The kit can detect less than 20 μM of Acetate in various samples. The standard curve ranges from 0 - 10 nmole/well.

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  4. What is the enzymatic reaction and the resulting intermediate substance?

    1 answer

    1. Initially, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. This intermediate then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The stated intermediate, considered proprietary, is NADH-based."

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