indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing HOXB4 western blot: 2-4 μg/mL using lysates of K562
Pricing and availability is not currently available.
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~35 kDa
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing HOXB4 western blot: 2-4 μg/mL using lysates of K562
Homeobox B4 (HOXB4) gene is mapped to human chromosome 17q21.
Application
Anti-HOXB4 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Hox genes helsp to modulate important development pathways that are involved in morphogenesis of the embryo. It also helps in the formation of the body plan during embryonic development. These gene are known to play important roles in normal development, altered homeobox gene function or expression. It is also implicated in the development of cancers, such as leukemias or neoplasms of the breast, prostate, kidney, colon, skin and brain. Homeobox B4 (HOXB4) helps to control the balance between hematopoietic stem cell renewal and differentiation. Suppression of HOXB4 may reverse multidrug resistance (MDR) of K562/ADM cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Role of homeobox genes in the patterning, specification and differentiation of ectodermal appendages in mammals
Duverger O, et al.
Journal of Cellular Physiology, 216(2), 337-337 (2008)
HOXB4 knockdown reverses multidrug resistance of human myelogenous leukemia K562/ADM cells by downregulating P-gp, MRP1 and BCRP expression via PI3K/Akt signaling pathway
Wang H, et al.
International Journal of Oncology, 49(6), 2529-2537 (2016)
Hematopoietic Expression of Hoxb4 Is Regulated in Normal and Leukemic Stem Cells through Transcriptional Activation of the Hoxb4 Promoter by Upstream Stimulating Factor (Usf)-1 and Usf-2
Wang H, et al.
The Journal of Experimental Medicine, 192(10), 1479-1479 (2000)
Homeobox genes in mammary gland development and neoplasia
Only a handful of cell types, including fibroblasts, epithelial, and endothelial cells, can support human cytomegalovirus (CMV) replication in vitro, in striking contrast to the situation in vivo. While the susceptibility of epithelial and endothelial cells to CMV infection is
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