GE27-5140-01

Microspin^™ S-400 HR

Cytiva 27-5140-01, pack of 50 columns

• Synonym(s): Microspin S-400 HR
• UNSPSC Code: 41105501
• NACRES: NA.52

Properties

• packaging: pack of 50 columns
• manufacturer/tradename: Cytiva 27-5140-01
• storage condition: (20% Ethanol); (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
• matrix: allyl dextran and N, N’-methylenebisacrylamide
• particle size: 25-75 μm
• avg. part. size: 50 μm
• cleaning: 2-13
• working range: 3-11
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

MicroSpin^™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity is obtained with sample volumes from 25-100 μl, and from nanogram to milligram quantities of DNA.

Features and Benefits

• illustra^™ MicroSpin^™ S-400 HR Columns: For rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography. - Accommodates 25 to 50 μ for post-PCR cleanup prior to cloning or a second amplification reaction or 51 to 100 μ for all other applications.
• Convenient: Prepacked with Sephacryl^® S-400 HR pre-equilibriated in TE buffer.
• Ready to use: Requires less than 4 min from sample application to collection of purified product.
• Tested in nickase, single- and double-stranded exonuclease and RNase assays.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MicroSpin is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

illustra is a trademark of Cytiva

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids