When considering your question, the initial response was that Fast Red TR is typically used for light microscopy. I consulted Conn's Biological Stains, 10th Edition from the Biological Stain Commission and found a reference for its use in fluorescence microscopy in an article by Lizard, G et al (1997). The article is titled "Detection of human papillomavirus DNA in genital lesion by enzymatic in situ hybridization with fast red and laser confocal microscopy" and was published in the Histochemical Journal (29: 544-554).
F4648
Fast Red TR/Naphthol AS-MX
SIGMAFAST™, fluorogenic, tablet
Synonym(s):
4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets
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Product Name
SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL
Quality Level
form
tablet
solubility
water: 1 tablet/mL, clear to hazy, colorless to faintly yellow
storage temp.
−20°C
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Application
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
- in alkaline phosphatase staining in organ sections[1]
- for staining chondrogenic spheroids in immunohistochemistry studies[2]
- for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections[3]
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
Reconstitution
Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Could product F4648 be used with a 543 laser system?
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