This product is a cell line supplied by ECACC. The supplier notes that all cell lines are handled at containment level 2 in accordance with the Advisory Committee on Dangerous Pathogens (ACDP). This cell line was established in 1990, see Proc Royal Soc (B) 1990;241:187. For additional information please see the link below to review the supplier information:
https://www.culturecollections.org.uk/nop/product/nd723
ND7/23 Cell Line
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090903, Mouse x Rat Hybridoma nerve, Neuronal
Synonym(s):
ND7/23 Cells
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
Mouse x Rat Hybridoma nerve
packaging
tube of 5 μg 92090903-DNA-5UG
pkg of vial of cells 92090903-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma x Rat neurone hybrid
Cell Line Description
Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.
Application
Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.
Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Guten Tag, gehen wir Richtig in der Annahme, dass die Zellen als S1 GVO eingestuft sind?
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