biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-CD1, bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG3
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, but not on peripheral blood mononuclear cells. The antigen recognised is the bovine equivalent of CD1.
Immunogen
Bovine thymocytes
Culture Medium
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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