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Product Name
CCRF-CEM, 85112105
biological source
human blood
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian acute lymphoblastic leukaemia
Cell Line Description
A T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 2-3x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.
Other Notes
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