PEO14

10032311, human ovary, epithelial; stellate, producing hemicystic colonies that detach as spheres

• Synonym(s): PE014
• UNSPSC Code: 41106514

Properties

• Product Name: PEO14, from human ovary, 10032311
• biological source: human ovary
• growth mode: Adherent
• karyotype: For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
• morphology: Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres.
• products: Not specified
• receptors: Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Description

Cell Line Origin

Human ovarian cancer; oestrogen receptor negative

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 11,12
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 15,16

Culture Medium

RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Doubling time approximately 108 hours.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.