C6357
Caspase 4 human
recombinant, expressed in E. coli, 5,000 units/mg protein
Synonym(s):
ICE(rel)II, Ich-2, TX
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About This Item
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
recombinant
expressed in E. coli
form
solution
specific activity
5,000 units/mg protein
mol wt
30 kDa
solubility
PBS: soluble
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CASP4(837)
General description
The caspase 4 (CASP4) gene is mapped to human chromosome 11q21. Caspase 4 is a cysteine-aspartic protease, localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).
Biochem/physiol Actions
Caspase 4 is an inflammatory caspase associated with innate immune responses. It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).
Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.
Unit Definition
One unit will hydrolyze 1 nmol of the caspase substrate WEHD-pNA to WEHD and p-nitroaniline per hour at pH 7.2 at 37 °C.
Physical form
Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Giuliana Papoff et al.
Scientific reports, 8(1), 17705-17705 (2018-12-12)
Inflammatory caspases, including human caspase-4 (CASP4), play key roles in innate immune responses to promote fusion of phagosomes harboring pathogenic bacteria with lysosomes, halt intracellular replication of pathogens, maturation and secretion of pro-inflammatory cytokines. The role of inflammatory caspases in
Itziar Salaverria et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 25(10), 1216-1222 (2007-02-14)
To compare the genetic relationship between cyclin D1-positive and cyclin D1-negative mantle cell lymphomas (MCLs) and to determine whether specific genetic alterations may add prognostic information to survival prediction based on the proliferation signature of MCLs. Seventy-one cyclin D1-positive and
Caspase-4 and Caspase-5
Handbook of Proteolytic Enzymes, 2265-2269 (2013)
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