C4237

Protamine Kinase, Cytosolic from bovine kidney

>90% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): Cytosolic Protamine Kinase
• MDL number: MFCD04041070
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 200
• Assay: >90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥15,000 units/mg protein
• mol wt: 45 kDa
• packaging: vial of 100 ng
• shipped in: dry ice
• storage temp.: −70°C

Description

Application

Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.

Biochem/physiol Actions

Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase [1].

Unit Definition

One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30 °C.

Physical form

Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol

Safety Information

• Storage Class Code: 10 - Combustible liquids