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A8181

Sigma-Aldrich

Amido Black Staining Solution 2X

electrophoresis reagent

Synonym(s):

Naphthol Blue Black solution, Amido Black 10B

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About This Item

Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
Molecular Weight:
616.49
Beilstein:
5374263
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32

form

liquid

SMILES string

[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:
  • serum proteins present in the culture supernatant
  • cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
  • histones from chromatin samples

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

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Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Meghan C Hughes et al.
The Journal of physiology, 598(7), 1377-1392 (2019-01-24)
Ninety-eight per cent of patients with Duchenne muscular dystrophy (DMD) develop cardiomyopathy, with 40% developing heart failure. While increased propensity for mitochondrial induction of cell death has been observed in left ventricle, it remains unknown whether this is linked to
Protein Blotting and detection: methods and protocols, 86(1), 10-18 (2009)
Placental release of taurine to both the maternal and fetal circulations in human term pregnancies
Holm MB, et al.
Amino Acids, 50(9), 1205-1214 (2018)
Detection of proteins on blot membranes
Goldman A, et al.
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 86(1), 10-18 (2016)
Adsorptive Behavior of Kaolin for Amido Black Dye in Aqueous Solution
Ayanda OS, et al.
Orient. J. Chem., 34(3), 1233-1239 null

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Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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