A1902
Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Rabbit Anti-Mouse IgG (whole molecule)−AP
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About This Item
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biological source
rabbit
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
mouse
technique(s)
direct ELISA: 1:1,000
western blot: suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.
Specificity
Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
Biochem/physiol Actions
IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Physical form
Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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