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76250

Sigma-Aldrich

Pararosaniline hydrochloride

for microscopy (Bact., Bot., Hist.)

Synonym(s):

Basic Fuchsin, Basic Parafuchsin, Basic Red 9, Magenta™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride

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About This Item

Linear Formula:
C19H18N3Cl
CAS Number:
569-61-9
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein:
4164603
EC Number:
209-321-2
MDL number:
MFCD00001657
UNSPSC Code:
12352200
PubChem Substance ID:
24886964

grade

for microscopy (Bact., Bot., Hist.)

suitability

suitable for microscopy

SMILES string

Cl.Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=N)C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

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Other Notes

This is the pure, principal component of mixtures commonly referred to as Basic Fuchsin.
Reagent for the colorimetric determination of sulfite in foods

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCBL4000V
BCBJ7085V
BCBH3921V
BCBG9372V
BCBB2243V

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S. Ogawa et al.
Zeitschrift fur Lebensmittel-Untersuchung Und -Forschung, 168, 298-298 (1979)
J A Kiernan
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 79(5-6), 203-210 (2005-03-15)
Hexazonium pararosaniline is a valuable reagent that has been used in enzyme activity histochemistry for 50 years. It is an aqueous solution containing the tris-diazonium ion derived from pararosaniline, an aminotriarylmethane dye, and it contains an excess of nitrous acid
K A Crist et al.
Journal of cellular biochemistry. Supplement, 25, 49-56 (1996-01-01)
Image analysis of tissue biopsies for determination of DNA content as an early marker of neoplasia is hampered by the complexity of corrections necessary to dea with nuclear truncation and overlap in thin sections. The use of confocal laser scanning
L Galassi
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 68(3), 175-179 (1993-05-01)
Formation of crystals in Schiff reagents prepared from SO2 gas previously has been reported either soon after preparation, using high dye concentrations and heating, or after long periods of storage at room temperature. With the first type of procedure only
I A Schrijver et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(1), 95-103 (2000-02-01)
Integral immunohistochemical analysis of immune responses in frozen sections requires that, in addition to constitutively expressed membrane CD markers, less stable determinants can be reliably visualized. Therefore, we compared the commonly used acetone fixation method with pararosaniline fixation for six
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