Skip to Content
Merck
All Photos(1)

Documents

42424

Sigma-Aldrich

Atto 550

for fluorescence, ≥90% (HPLC)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD05865402
UNSPSC Code:
12352108
NACRES:
NA.32

grade

for fluorescence

Assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 553-559 nm Amax

storage temp.

−20°C

General description

Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3® and is characterized by a high photostability.

find more information here

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3™, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
CAB-O-SIL is a registered trademark of Cabot Corp.
Cy3 is a trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 5

1 of 5

Sigma-Aldrich

Sigma-Aldrich

97875

Atto 647

Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

70425

Atto 590

Atto 647N BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Supelco

04507

Atto 647N

Atto 700 BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Sigma-Aldrich

30674

Atto 700

NTA - Atto 488 BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)

Supelco

39625

NTA - Atto 488

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Rula Masoud et al.
ACS nano, 6(7), 6272-6283 (2012-06-06)
We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy
Rahul Roy et al.
Nature methods, 5(6), 507-516 (2008-05-31)
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by
A molecular toolkit for population genetic investigations of the ash dieback pathogen Hymenoscyphus pseudoalbidus.
Gross, A., et al.
Forest Pathology, 42, 252-264 (2012)
View All Related Papers

Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service