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X4251

Supelco

XTT sodium salt

analytical standard, ≥90%

Synonym(s):

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt

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About This Item

Empirical Formula (Hill Notation):
C22H16N7NaO13S2
CAS Number:
111072-31-2
Molecular Weight:
673.52
MDL number:
MFCD00083517
UNSPSC Code:
41116107
PubChem Substance ID:
329830806
NACRES:
NA.24
Pricing and availability is not currently available.

grade

analytical standard

Quality Level

100

Assay

≥90%

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

forensics and toxicology
pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

[Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1

InChI key

JACYMBNQPPWQML-UHFFFAOYSA-M

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Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Useful in AIDS and cancer testing.[1]
XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Principle

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H242

Hazard Classifications

Self-react. C

Supplementary Hazards

EUH044

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCB8867
SLCB3746
SLCB3752
SLCB3757
SLBZ5370

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Parsons, J.L., et al.
Journal of Heterocyclic Chemistry, 25, 911-911 (1988)
Wander José da Silva et al.
Brazilian dental journal, 19(4), 364-369 (2009-01-31)
2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm
D A Scudiero et al.
Cancer research, 48(17), 4827-4833 (1988-09-01)
We have previously described the application of an automated microculture tetrazolium assay (MTA) involving dimethyl sulfoxide solubilization of cellular-generated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-formazan to the in vitro assessment of drug effects on cell growth (M.C. Alley et al., Proc. Am. Assoc.
Cristina Sánchez-Quesada et al.
Evidence-based complementary and alternative medicine : eCAM, 2018, 9473094-9473094 (2018-10-27)
Anti-inflammatory effects of virgin olive oil (VOO) have been described recently, along with its wound healing effect. One of the main minor compounds found in VOO is squalene (SQ), which also possesses preventive effects against skin damage and anti-inflammatory properties.

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