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R1273

Millipore

Rose bengal agar base

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

pH

7.2±0.2 (25 °C)

application(s)

bioburden testing
clinical testing
environmental
food and beverages
microbiology

suitability

selective for Aspergillus spp.
selective for Candida spp.
selective for Penicillium spp.
selective for Pichia spp.
selective for Saccharomyces spp.
selective for Zygosaccharomyces spp.
selective for molds (General Media)
selective for yeasts (General Media)

Application

Ingredients (g/L) for the selective isolation and enumeration of yeasts and molds.

Components

Ingredients (g/L)
Papic digest of soybean meal, 5.00
Dextrose, 10.00
Monopotassium phosphate, 1.00
Magnesium sulphate, 0.50
Rose bengal, 0.05
Agar, 15.00

Preparation Note

Suspend 31.55 g of Rose Bengal Agar Base in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45°C and add 2 vials of rehydrated Chloramphenicol Selective Supplement (Cat. No. 29231) to 1 litre of the Rose Bengal Agar Base. Mix thoroughly and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL3686
BCCL5436
BCCM1991
BCCK9379
BCCL0747

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Comparison of an improved rose bengal-chlortetracycline agar with other media for the selective isolation and enumeration of moulds and yeasts in foods.
B Jarvis
The Journal of applied bacteriology, 36(4), 723-727 (1973-12-01)

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Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

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Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

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