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A4628

Sigma-Aldrich

Bovine Serum Albumin solution

5% in 0.85% sodium chloride, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

300

sterility

aseptically filled

Assay

4.8-5.5% protein basis (biuret)

form

solution

purified by

heat shock fractionation

does not contain

stabilizer or preservative

origin

USA origin

concentration

4.8-5.5% (biuret)
5% in 0.85% sodium chloride

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin, a large molecular weight protein, accounts for around 10% of total whey proteins. It is a source of essential amino acids and is the third most abundant whey protein found in milk. BSA is biodegradable, biocompatible, nontoxic, and nonimmunogenic.

Application

Bovine Serum Albumin solution has been used:
  • as a component in Opti-minimum essential medium (MEM) to serve as the transfection media to study the effect of albumin on transfection efficiency
  • as a component in 0.1% Tween-20/PBS (PBST) in diluting primary antibodies to incubate cells
  • to incubate hydroxyapatite (HA) for in vitro study

Biochem/physiol Actions

Bovine serum albumin is capable of binding free fatty acids, other lipids, and flavor compounds. Because of its low cost, tolerance, and ease of preparation, BSA has been widely used in drug delivery. BSA can be used as a colloidal delivery system. It is also appealing as a nanoparticle matrix due to its low cost, ease of purification, and aqueous solubility. BSA nanoparticles can load several drugs and bioactive compounds due to drug binding sites in the BSA molecule.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000394612
0000380974
0000376373
0000371540
0000366398

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Heterogeneity, Fractionation, and Isolation
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G Daculsi et al.
Journal of biomedical materials research, 47(2), 228-233 (1999-08-17)
The role of adhesion molecules like osteopontin and bone sialoprotein, both containing the Arg-Gly-Asp sequence have been shown to have a role in mineral formation, whereas fibronectin (FN), another adhesive protein, was never studied during the mineralization processes. The formation
Whey proteins in functional foods
3D Printing and Biofabrication, 637-663 (2019)
Qiang Lou et al.
American journal of physiology. Cell physiology, 316(1), C81-C91 (2018-11-09)
RNA-induced silencing complex (RISC) is formed during RNA interference (RNAi), whereas stress granules (SG) are assembled in response to cellular stress. Here, we demonstrate an interesting connection between RISC and SG that may involve argonaute 2 (Ago2), a core component
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