88151
Tetrathionate Broth
suitable for microbiology, NutriSelect® Plus
Synonym(s):
TB, TT Broth
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About This Item
UNSPSC Code:
41171607
NACRES:
NA.74
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
bile salts, 1 g/L
calcium carbonate, 10 g/L
mixed peptone, 5 g/L
sodium thiosulfate, 30 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
7.8±0.2 (25 °C)
application(s)
environmental
food and beverages
pathogen testing
sample preparation
microbiology
suitability
selective for Salmonella spp.
Application
For the selective enrichment of Salmonella from various sources.
Preparation Note
Dissolve 46 g in 1 litre distilled water and heat carefully till boiling (do NOT sterilize). Cool to below 45°C then add 20 ml iodide solution (5 g potassium iodide (Cat. No. 60400) and 6 g iodine (Cat. No. 57650) in 20 ml distilled water) and 10 ml 0.1% brilliant green (Cat. No. 16020; 10 mg/l) solution. Mix and fill containers so that any insoluble material is evenly distributed. This broth should be prepared on the day of use.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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L. Muller
Comp. rend. Soc. biol., 89, 434-434 (1923)
Novobiocin-tetrathionate broth: a medium of improved selectivity for the isolation of Salmonellae from faeces.
L JEFFRIES
Journal of clinical pathology, 12, 568-571 (1959-11-01)
Stefanie Mangold et al.
Frontiers in microbiology, 2, 17-17 (2011-06-21)
Given the challenges to life at low pH, an analysis of inorganic sulfur compound (ISC) oxidation was initiated in the chemolithoautotrophic extremophile Acidithiobacillus caldus. A. caldus is able to metabolize elemental sulfur and a broad range of ISCs. It has
S A Palumbo et al.
Applied microbiology, 20(6), 970-976 (1970-12-01)
Tetrathionate enrichment broth is a complex mixture of salts including iodides and other polythionates, but only thiosulfate (0.0736 m) and tetrathionate (0.0236 m) in combination were toxic for Escherichia coli. Individually, these two salts were not lethal. The lethal action
Kauffmann, F.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale, 117, 26-26 (1935)
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