Aniline Blue is commonly used as a collagen stain, but there is no specific mention of its use to stain glucans.Aniline Blue can be specifically used to detect (1,3)-β-glucans. For the specific pH 8 solution formulation for staining with Aniline Blue, it is suggested to use a dilute solution of ammonium hydroxide or a dilute solution of sodium hydroxide. Further searching is recommended to provide an actual staining procedure.
415049
Aniline Blue diammonium salt
certified by the Biological Stain Commission
Synonym(s):
Ink Blue R; 4-[4-[[4-(4-aminooxysulfonylanilino)phenyl]-[4-(4-aminooxysulfonylphenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonic acid
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All Photos(3)
About This Item
Empirical Formula (Hill Notation):
C37H32N5O9S3
CAS Number:
Molecular Weight:
786.87
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
grade
certified by the Biological Stain Commission
Quality Level
form
powder, crystals or granules
composition
Dye content, 65%
color
brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown
ε (extinction coefficient)
15000 at 595-605 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[S](=O)(=O)(ON)c1ccc(cc1)Nc2ccc(cc2)C(=C5C=CC(=Nc6ccc(cc6)[S](=O)(=O)ON)C=C5)c3ccc(cc3)Nc4ccc(cc4)[S](=O)(=O)O
InChI
1S/C37H31N5O9S3/c38-50-53(46,47)35-21-15-32(16-22-35)41-29-9-3-26(4-10-29)37(25-1-7-28(8-2-25)40-31-13-19-34(20-14-31)52(43,44)45)27-5-11-30(12-6-27)42-33-17-23-36(24-18-33)54(48,49)51-39/h1-24,40-41H,38-39H2,(H,43,44,45)
InChI key
CSYADFXDZYPANK-UHFFFAOYSA-N
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General description
Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. It is a mixture of methyl blue (Cl 42780, Acid blue 93) and water blue (Cl 42755, Acid blue 22).
Application
Aniline blue (nominally as methyl blue or water blue) is a component of commonly used polychrome stains. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.
Suitability
Certified for use in Mallory′s connective tissue stain and Gomori′s one-step trichrome.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Can item 415049, Aniline Blue diammonium salt, be used to stain glucan G6513?
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