Biochimica et biophysica acta, 898(3), 331-337 (1987-04-23)
The free Mg2+ concentration within synaptosomes has been measured with an entrapped Mg2+ indicator, eriochrome blue. Ionophores gramicidin and A23187 slowly increased the absorbance of the entrapped dye. Calibration of the dye response in a Na+-based medium gave a value
Measurement of ionized magnesium in biological fluids.
A nondestructive technique is described for measuring intracellular free Mg2+ concentrations. It is based on the microinjection of a Mg2+ metallochromic indicator in the cytosol of a large single cell and measuring its differential absorbance changes with sensitive multiple wavelength
Cytoplasmic, free Mg2+ was measured spectrophotometrically using an intrasynaptosomally entrapped Mg2+-indicator, Eriochrome blue (EB). Addition of the ionophore A23187 or disruption of the synaptosomal plasma membrane with digitonin caused an increase in absorbance of entrapped EB with a maximum at
Intracellular free Mg2+ (Mgf) in darkness and during illumination was measured in the Balanus photoreceptor using the Mg2+ sensitive dye Eriochrome Blue SE (EB). The EB was pressure injected in the cell and Mgf determined from measurements of absorption changes
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