11945
Benedict′s reagent
for the qualitative determination of sugars
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Quality Level
technique(s)
photometry: suitable
impurities
cupric sulfate
application(s)
food and beverages
general analytical
InChI
1S/C6H8O7.CH2O3.Cu.2Na.H2O4S.H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;2-1(3)4;;;;1-5(2,3)4;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);(H2,2,3,4);;;;(H2,1,2,3,4);1H2/q;;+2;2*+1;;/p-4
InChI key
QFKWSRIUZIYLCK-UHFFFAOYSA-J
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General description
Benedict′s reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.
Application
Benedict′s reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites. It may also be also be used to synthesize an SEM image of the cubic shaped Cu2O with smooth surface.
Other Notes
Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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R.L. Shriner, et al.
The Systematic Identification of Organic Compounds (1980)
Textbook of Medical Biochemistry.
Ramakrishnan S
Science, 18-19 (2004)
[Caustic lesions caused by test tablets used in semiquantitative urinary sugar determination (Clinitest)].
I Schlatter-Lanz
Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis, 74(50), 1402-1406 (1985-12-10)
Fatal accidental ingestion of Clinitest in adult.
H J O'Connor et al.
Journal of the Royal Society of Medicine, 77(11), 963-965 (1984-11-01)
Dangers of Clinitest ingestion.
A Barge et al.
Journal of the Royal Society of Medicine, 78(3), 271-271 (1985-03-01)
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